Position Description

The technician’s daily responsibilities include tissue culture to maintain glioma cell lines and patient-derived glioma neurospheres, validate the genetic hits from the CRISPR screen by conducting growth curve of the cell lines/patient-derived glioma neurospheres, cloning and viral packaging to create virus for genetic perturbation, confirm genetic perturbation of the cells/tissues by performing RT-PCR and Western blot, perform intracranial injection of glioma cell lines/patient-derived glioma neurospheres and monitor animal health and the tumor growth by animal imaging, perform mouse brain perfusion, dissection and slicing, perform RNA in situ hybridization and immunohistochemistry to assess the spatial protein expression, perform imaging acquisition and analysis, perform data analysis and graphing. The technician will spend 50% of the time at Sabatini lab and 50% of the time at Haigis lab every week, participate lab meetings in both labs and fulfill lab responsibilities from both labs. The technician will learn and perform experiments with Dr. Annie Hsieh side by side and meet with her weekly to discuss experimental plan and data interpretation.

Prior laboratory experience is required, as well as strong organizational skills and an eagerness to learn. Successful candidates will be independent, outgoing, flexible, self-motivated, and able to assimilate new information and execute quickly. You will have the opportunity to direct the research project while taking advantage of the intelligent, diverse, welcoming, and exciting team of researchers at both the Sabatini lab and Haigis lab.

Basic Qualifications

One-year related lab experience. Advanced education may count towards experience.

Additional Qualifications and Skills

• Bachelor's or Master’s degree with prior experience in a wet lab environment. A major in Neuroscience, Biology, and Chemistry is preferred.
• Excellent organizational and communication skills are required.
• Experience in one or more of the following techniques would be preferred: cell culture, biochemistry techniques, mouse intracranial surgery, and histological preparations.
• Experience or willingness to work with mice, manage a mouse colony and perform survival and non-survival procedures as necessary.
• Analytical skills to resolve problems that require the use of basic scientific, mathematical, or technical principles. Some experience with programming, using R and/or Python is preferred.
• Candidates should be communicative with excellent interpersonal skills, attentive to detail, organized, and thoughtful about the protocols and techniques being executed
• Creativity, flexibility, and perseverance to troubleshoot and overcome inevitable experimental difficulties and changing experimental goals in light of new results.

Additional Information

This is a one-year term position from the date of hire, with the possibility of extension, contingent upon work performance and continued funding to support the position. 

This is an in-person position based on our campus in Boston.

Offer contingent upon successful completion of a pre-placement medical evaluation.

The University requires all Harvard community members to be fully vaccinated against COVID-19 and remain up to date with COVID-19 vaccine boosters, as detailed in Harvard’s Vaccine & Booster Requirements. Individuals may claim exemption from the vaccine requirement for medical or religious reasons. More information regarding the University’s COVID vaccination requirement, exemptions, and verification of vaccination status may be found at the University’s “COVID-19 Vaccine Information” webpage: http://www.harvard.edu/coronavirus/covid-19-vaccine-information/. 

Please note that we are currently conducting a majority of interviews and onboarding remotely and virtually. We appreciate your understanding.

Harvard University offers an outstanding benefits package including:

• Time Off: 3 - 4 weeks paid vacation, paid holiday break, 12 paid sick days, 12.5 paid holidays, and 3 paid personal days per year.
• Medical/Dental/Vision: We offer a variety of excellent medical plans, dental & vision plans, all coverage begins as of your start date.
• Retirement: University-funded retirement plan with full vesting after 3 years of service.
• Tuition Assistance Program: Competitive tuition assistance program, incredibly affordable classes directly at the Harvard Extension School, and discounted options through participating Harvard grad schools.
• Transportation: Harvard offers a 50% discounted MBTA pass as well as additional options to assist employees in their daily commute.
• Wellness Options: Harvard offers programs and classes at little or no cost, including stress management, massages, nutrition, meditation, and complementary health services.
• Harvard access to athletic facilities, libraries, campus events, and many discounts throughout metro Boston.

The Harvard Medical School is not able to provide visa sponsorship for this position.

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Commitment to Equity, Diversity, Inclusion, and Belonging

We are committed to cultivating an inclusive workplace culture of faculty, staff, and students with diverse backgrounds, styles, abilities, and motivations. We appreciate and leverage the capabilities, insights, and ideas of all individuals. Harvard Medical School Mission and Community Values

EEO Statement

We are an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability status, protected veteran status, gender identity, sexual orientation, pregnancy and pregnancy-related conditions, or any other characteristic protected by law.